Identification and Characterization of the Pathogen Causing Canker Disease in Acid Lime (Citrus aurantiifolia) cv. Petlur Acid Lime
Pothuraju Pradeep Kumar *
Dr. Y. S. R Horticultural University, College of Horticulture, Venkataramannagudem, India.
C. Madhumathi
Citrus Research Station, Petlur, India.
M. Madhavi
College of Horticulture, Venkataramannagudem, India.
B. Tanuja Priya
Department of Horticulture, Dr. Y. S. R Horticultural University, Lam, Guntur, India.
M. Paratpara Rao
College of Horticulture, Venkataramannagudem, India.
M. Kavitha
Citrus Research Station, Tirupathi, India.
*Author to whom correspondence should be addressed.
Abstract
In this study, the pathogen responsible for canker disease in Acid Lime Cv. Petlur Acid Lime was identified through a comprehensive analysis involving both morphological and molecular characterization techniques. Cultures of the Xanthomonas campestris pv. citri (Xcc) bacterial isolate, grown on artificial media, exhibited distinct characteristics: small, round, and mucoid colonies with a gram-negative nature, as revealed by Gram staining. Molecular identification was performed using universal primers 27F and 1492R, resulting in a specific DNA band of 424 bp, confirming the presence of the pathogen in the samples.
Keywords: Citrus canker, xanthomonas campestris pv. citri, acid lime, morphological characterization, molecular identification