An Effective Protocol for Carnation (Dianthus caryophyllus L.) cv. 'Geolei' Explants Sterilization for Successful Callusing and Shoot Regeneration
Ujjwal Sirohi *
Department of Agricultural Biotechnology,Sardar Vallabhbhai Patel University of Agricultureand Technology, Meerut, 250110, Uttar Pradesh, India.
Swati Sharma
Department of Agricultural Biotechnology,Sardar Vallabhbhai Patel University of Agricultureand Technology, Meerut, 250110, Uttar Pradesh, India.
Mukesh Kumar
Department of Horticulture,Sardar Vallabhbhai Patel University of Agricultureand Technology, Meerut, 250110, Uttar Pradesh, India.
R. S. Sengar
Department of Agricultural Biotechnology,Sardar Vallabhbhai Patel University of Agricultureand Technology, Meerut, 250110, Uttar Pradesh, India.
L. K. Gangwar
Department of Genetics and Plant Breeding,Sardar Vallabhbhai Patel University of Agricultureand Technology, Meerut, 250110, Uttar Pradesh, India.
Akash Tomar
College of Biotechnology, Sardar Vallabhbhai Patel University of Agricultureand Technology, Meerut, 250110, Uttar Pradesh, India.
Vyankatesh Dhanraj Bagul
Department of Agricultural Biotechnology,Sardar Vallabhbhai Patel University of Agricultureand Technology, Meerut, 250110, Uttar Pradesh, India.
M. K. Yadav
Department of Agricultural Biotechnology,Sardar Vallabhbhai Patel University of Agricultureand Technology, Meerut, 250110, Uttar Pradesh, India.
*Author to whom correspondence should be addressed.
Abstract
Carnation is a popular floricultural crop grown widely for its attractive cut flowers. Micro-propagation can be used to create large-scale carnation output. For growth and development, plants require some necessary nutrients as well as growth regulators. Due to the importance of carnation, the present work is carried out using leaf and nodal segments to examine the potential of several plant growth regulators for in vitro callus formation and adventitious shoot regeneration. Explants were sterilized properly with bavistin, sodium hypochlorite and mercuric chloride. The minor contaminated cultures were created by consecutively treating the explants with 0.25% bavistin, 0.50% sodium hypochlorite, and 0.1% mercuric chloride for ten, fifteen, and two minutes.
MS media with 2.5 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) in combination with 0.75 mg/l naphthalene acetic acid (NAA) resulted in the maximum callus induction (90.47%) from leaf explants. Maximum shoots (76.47%) were produced in MS media supplemented with 2.0 mg/l Thidiazuron (TDZ) + 0.25 mg/l NAA. NAA at 1.25 mg/l was most efficient for maximum root induction (83.32%). In the present study, an effective protocol of carnation explants sterilization was optimized for successful callusing and shoot regeneration.
Keywords: Bavistin, callus induction, 2,4-dichlorophenoxy acetic, naphthalene acetic acid, mercuric chloride, regeneration, sodium hypochlorite, thidiazuron